Western blot analysis of CACNA1B in extracts of U-87MG cells. Samples were incubated with CACNA1B Polyclonal antibody (Product # PA5-109473) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3 min.